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chemidoc xrs imaging system  (Bio-Rad)


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    Structured Review

    Bio-Rad chemidoc xrs imaging system
    Chemidoc Xrs Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 34698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chemidoc+imagers/ChemiDoc+Imaging+System/pmc12811436-69-13-17
    Average 99 stars, based on 34698 article reviews
    chemidoc xrs imaging system - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Implications of hippocampal excitatory amino acid transporter 2 in modulating anxiety and visceral pain in a mouse model of inflammatory bowel disease.
    Article Snippet: Inflammatory bowel disease (IBD) is characterized by chronic inflammation and significantly impairs quality of life through anxiety-like behaviors and visceral pain.. Early evaluation of the risk of anxiety-like behaviors and visceral pain in IBD patients, along with targeted treatment, may benefit disease management.. Visceral pain and anxiety-like behavior are often accompanied by neurological damage.

    Article Title: Investigating the mechanism of ferroptosis induction by sappanone A in hepatocellular carcinoma: NRF2/xCT/GPX4 axis.
    Article Snippet: Hepatocellular carcinoma (HCC) is a prevalent and lethal malignancy with significant global impact, necessitating the development of novel therapeutic strategies and drugs.. Ferroptosis, a newly identified form of irondependent programmed cell death, has emerged as a promising strategy to combat HCC.. Sappanone A, an isoflavone compound derived from the heartwood of Biancaea sappan (L.) Tod., is known for its anti-inflammatory and antioxidant properties.

    Article Title: Pharmacological targeting of the mitochondrial phosphatase PTPMT1 sensitizes hepatocellular carcinoma to ferroptosis
    Article Snippet: In this study, the primary antibodies employed were as follows: anti-GPX4 (1:1000; Proteintech, 67763-1-Ig), anti-SLC7A11 (1:1000; Proteintech, 26864-1-AP), anti-FSP1 (1:1000; Proteintech, 68049-1-Ig), anti-DHODH (1:2000; Proteintech, 14877-1-AP), anti-TUBULIN (1:3000; Proteintech, 11224-1-AP), anti-FTL (1:2000; Proteintech, 10727-1-AP), anti-ATG5 (1:2000; Proteintech, 10181-2-AP), anti-BECLIN1 (1:2000; Proteintech, 11306-1-AP), anti-p62 (1:1000; Proteintech, 18420-1-AP), anti-LC3 (1:1000; Proteintech, 14600-1-AP), anti-MFN1 (1:2000; Proteintech, 13798-1-AP), anti-TOM20 (1:2000; Proteintech, 11802-1-AP), anti-PINK (1:2000; Proteintech, 23274-1-AP), anti-PGC-1α (1:2000; Proteintech, 66369-1-Ig), anti-SDHA (1:2000; Proteintech, 14865-1-AP), anti-NRF2 (1:1000; Proteintech, 16396-1-AP), anti-CASPASE3 (1:1000; ABclonal, A2156), anti-BCL2 (1:1000; ABclonal, A0208) and anti-GAPDH (1:3000; Proteintech, 10494-1-AP). .. Following incubation with HRP-labeled secondary antibodies (ABclonal), protein bands were visualized utilizing an enhanced chemiluminescence kit (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Fisher) and ChemiDoc Imagers (Bio-Rad Laboratories, USA). ..

    Control:

    Article Title: Investigating the mechanism of ferroptosis induction by sappanone A in hepatocellular carcinoma: NRF2/xCT/GPX4 axis.
    Article Snippet: Hepatocellular carcinoma (HCC) is a prevalent and lethal malignancy with significant global impact, necessitating the development of novel therapeutic strategies and drugs.. Ferroptosis, a newly identified form of irondependent programmed cell death, has emerged as a promising strategy to combat HCC.. Sappanone A, an isoflavone compound derived from the heartwood of Biancaea sappan (L.) Tod., is known for its anti-inflammatory and antioxidant properties.

    Concentration Assay:

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation
    Article Snippet: .. Hydroxylamine was added to the supernatants to be final concentration of 0.5 M and the samples were boiled for another 5 min. Alk14 label signals were assessed by SDS-PAGE and in-gel fluorescence analysis with ChemiDoc Imagers (Bio-Rad). .. For Alk14 labeling of NLRP3 in BMDMs and THP-1 cells, after LPS priming, inflammasome activation and Alk14 incubation, 1×10 7 BMDMs were lysed with 0.6 mL 4% SDS buffer (4% SDS, 150 mM NaCl, 50 mM TEA, pH7.4) containing protease inhibitors and nuclease (Pierce), to make sure that all the NLRP3 protein (including membrane-bound NLRP3) was obtained after cell lysis.

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation.
    Article Snippet: .. Hydroxylamine was added to the supernatants to be final concentration of 0.5 M and the samples were boiled for another 5 min. Alk14 label signals were assessed by SDS-PAGE and in-gel fluorescence analysis with ChemiDoc Imagers (Bio-Rad). .. Cell Reports 43, 114070, April 23, 2024 21 For Alk14 labeling of NLRP3 in BMDMs and THP-1 cells, after LPS priming, inflammasome activation and Alk14 incubation, 13107 BMDMs were lysed with 0.6 mL 4% SDS buffer (4% SDS, 150 mM NaCl, 50 mM TEA, pH7.4) containing protease inhibitors and nuclease (Pierce), to make sure that all the NLRP3 protein (includingmembrane-bound NLRP3) was obtained after cell lysis.

    SDS Page:

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation
    Article Snippet: .. Hydroxylamine was added to the supernatants to be final concentration of 0.5 M and the samples were boiled for another 5 min. Alk14 label signals were assessed by SDS-PAGE and in-gel fluorescence analysis with ChemiDoc Imagers (Bio-Rad). .. For Alk14 labeling of NLRP3 in BMDMs and THP-1 cells, after LPS priming, inflammasome activation and Alk14 incubation, 1×10 7 BMDMs were lysed with 0.6 mL 4% SDS buffer (4% SDS, 150 mM NaCl, 50 mM TEA, pH7.4) containing protease inhibitors and nuclease (Pierce), to make sure that all the NLRP3 protein (including membrane-bound NLRP3) was obtained after cell lysis.

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation.
    Article Snippet: .. Hydroxylamine was added to the supernatants to be final concentration of 0.5 M and the samples were boiled for another 5 min. Alk14 label signals were assessed by SDS-PAGE and in-gel fluorescence analysis with ChemiDoc Imagers (Bio-Rad). .. Cell Reports 43, 114070, April 23, 2024 21 For Alk14 labeling of NLRP3 in BMDMs and THP-1 cells, after LPS priming, inflammasome activation and Alk14 incubation, 13107 BMDMs were lysed with 0.6 mL 4% SDS buffer (4% SDS, 150 mM NaCl, 50 mM TEA, pH7.4) containing protease inhibitors and nuclease (Pierce), to make sure that all the NLRP3 protein (includingmembrane-bound NLRP3) was obtained after cell lysis.

    Fluorescence:

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation
    Article Snippet: .. Hydroxylamine was added to the supernatants to be final concentration of 0.5 M and the samples were boiled for another 5 min. Alk14 label signals were assessed by SDS-PAGE and in-gel fluorescence analysis with ChemiDoc Imagers (Bio-Rad). .. For Alk14 labeling of NLRP3 in BMDMs and THP-1 cells, after LPS priming, inflammasome activation and Alk14 incubation, 1×10 7 BMDMs were lysed with 0.6 mL 4% SDS buffer (4% SDS, 150 mM NaCl, 50 mM TEA, pH7.4) containing protease inhibitors and nuclease (Pierce), to make sure that all the NLRP3 protein (including membrane-bound NLRP3) was obtained after cell lysis.

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation.
    Article Snippet: .. Hydroxylamine was added to the supernatants to be final concentration of 0.5 M and the samples were boiled for another 5 min. Alk14 label signals were assessed by SDS-PAGE and in-gel fluorescence analysis with ChemiDoc Imagers (Bio-Rad). .. Cell Reports 43, 114070, April 23, 2024 21 For Alk14 labeling of NLRP3 in BMDMs and THP-1 cells, after LPS priming, inflammasome activation and Alk14 incubation, 13107 BMDMs were lysed with 0.6 mL 4% SDS buffer (4% SDS, 150 mM NaCl, 50 mM TEA, pH7.4) containing protease inhibitors and nuclease (Pierce), to make sure that all the NLRP3 protein (includingmembrane-bound NLRP3) was obtained after cell lysis.



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